5. Ken Tokuhara, Ph.D.
Okinawa Churashima Foundation RESEARCH INSTITUTE

Research on subtropical plants

Ken Tokuhara, Ph.D.

Ken Tokuhara

Ken Tokuhara, Ph.D.

Area of
expertise		 Plant Cell Biotechnology
Research
content
  • Establishments of virus-free plants using tissue culture in tropical plants
  • Conservation and propagation of indigenous and endemic plants in Okinawa using tissue culture

Background

  • 2017 - Current post at the Okinawa Churashima Foundation Research Institute
  • 2004 - Ph.D. (Agriculture) Graduate school of Science and Technology, Chiba University
  • 1976 - B Sc. (Biology), Faculty of Science Toho University

Awards received

  • 2020 Conservation & Cultivation Award, Japan Association of Botanical Gardens

Research papers, Essays, Reports

  • Ken Tokuhara, Hiroyuki Sato, Atushi Abe, Kento Rammitsu, Yuki Ogura-Tsujita, Masahiro Mii. 2024. Seed germination and protocorm development of Platanthera stenoglossa Hayata subsp. iriomotensis (Masam.) K. Inoue (Orchidaceae) in both asymbiotic and symbiotic cultures. Biological Magazine of Okinawa 62: 7-16
  • Ken Tokuhara, Hiroyuki Sato, Atushi Abe, Masahiro Mii. 2023. In vitro optimization of seed germination and protocorm development in Gastrochilus japonicus (Makino) Schltr. (Orchidaceae), Plant Growth Regulation 101: 693-702
  • Amano, M., Kawamoto, N., Mine, Y., Tokuhara, K., Sato, H., Ogura-Tsujita, Y, 2023. In situ seed sowing experiment of Eulophia graminea (Orchidaceae) in Ocean Expo Park, Okinawa. Bull Jpn Assoc Bot Gard 57: 38-42 (in Japanese)
  • Liyue ZHANG, Kento RANMMITSU, Akihiko KINOSHITA, Ken TOKUHARA, Tomohisa YUKAWA, Yuki OGURA-TSUJITA (2022) Symbiotic Culture of Three Closely Related Dendrobium Species Reveals a Growth Bottleneck and Differences in Mycorrhizal Specificity at Early Developmental Stages Diversity 14(12) 1119
  • Hiroyuki SATO, Kensei AKAI, Ken TOKUHARA, Hana UMEMOTO, San’ei ICHIKAWA, Atsushi ABE. 2019. Limiting temperature on the survival of Diploprora championii（Orchidaceae）seedlings in Amami-Oshima Island, Japan. Bulletin of Japan Association of Botanical Gardens 54: 13-17.
  • Shrestha B. R., D. P. Chin, K. Tokuhara and M. Mii (2010). Agrobacterium-mediated transformation of Vanda using protocorm-like bodies. AsPac J. Mol. Biol. Biotechnol. 18:225-228.
  • Shrestha B. R., K. Tokuhara and M. Mii (2007). Plant regeneration from cell suspension-derived protoplast of Phalaenopsis. Plant Cell Rep. 26:719-725.
  • Tokuhara K., M. Mii (2003). Highly-efficient somatic embryogenesis from cell suspension cultures of Phalaenopsis orchid by adjusting carbohydrate sources. In Vitro Cell Dev., Biol.-Plant 39:635-639.
  • Tokuhara K., M. Mii (2001). Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In vitro Cell. Dev. Biol.-Plant. 37: 457-461.
  • Tsukazaki H., M. Mii, K. Tokuhara and K. Ishikawa (2000). Cryopreservation of Doritaenopsis suspension culture by vitrification. Plant Cell Rep., 19:1160-1164.
  • Tokuhara K., M. Mii (1998). Somaclonal variations in flower and inflorescence axis in microporagated plants through flower stalk bud culture of Phalaenopsis and Doritaenopsis. Plant Biotechnol. 15:23-28.
  • Tokuhara K., M. Mii (1993). Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalks buds. Plant Cell Rep., 13:7-11.

 

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